FIGURE 1.

Striatin is a scaffolding subunit within STRIPAK. A, composition of core STRIPAK. Proteins, paralogous genes (human nomenclature), and function of proteins are listed. B, schematic of the constructs used in this study. Human STRN3 constructs are in green, mouse Strn constructs are in purple. The first and last amino acid in each construct are indicated. C, summary of AP-MS results for the association of core STRIPAK components with the STRN3 (green) and Strn (purple) deletion mutants (top row). Identified proteins (hits; bottom row) in red do not interact with Strn(91–780) or STRN3(220–713), whereas hits in yellow do. The thickness of each line is proportional to the number of spectral counts (total number of peptides) recovered for each of the proteins in the analysis of the striatin mutants, relative to the spectral counts for the same protein in the AP-MS of full-length Strn. Note that each node (and its associated edges) represents paralogous families, as defined in A. The complete mass spectrometry data used to make this figure are presented in supplemental Table 1.