TABLE 2.

Nanometer-sized particle concentration and mean size for mAb1 stress-treated samples by nanoparticle tracking analysis

Stress treatment	Particle conc.a	Meanb	S.D.b
	E7/ml	nm	nm
bufferc	4	106	21
buffer-so+c	6	115	35
untreated	59	145	40
ft-slow	19	181	12
pH 11	38	120	35
agitate-so+	407	180	112
syringe-so+	597	149	44
stir-20h	642	217	60
stir-3d	38	118	49
H2O2	24	149	34
metal	216	156	46
65C/pH 8.5	109	97	24
90C	29	124	35

^a Concentration of particles in E7/ml was measured by nanoparticle tracking analysis.

^b Mean ± S.D. was calculated by the NanoSight NTA 2.0 software. See under “Experimental Procedures” for details.

^c A pH 5 buffer with silicone oil (buffer-so⁺) and without (buffer) was tested for nanoparticles.