FIGURE 8.

Glucose-induced down-regulation of glucose-6-phosphate dehydrogenase in ΔaqpZ cells. A, Western blot analyses of OpcA, a protein essential for glucose-6-phosphate dehydrogenase activity in the pentose phosphate pathway in protein extracts from WT and ΔaqpZ cells (top). The cells were exposed to 5 mm glucose for 48 h prior to extraction of proteins. Each lane was loaded with 3 μg of protein. Total protein (CBB) served as a loading control (bottom). B, the relative expression level of AqpZ protein was calculated by densitometry in relation to the sample without glucose. Data are mean ± S.D. (error bars) of values from three independent experiments.