FIGURE 2.

hnRNP M enhances exon 6 exclusion. His-tagged hnRNP M was overexpressed in the presence or absence of an antisense hnRNP M-expressing vector (AS-hnRNP M) in NIH3T3 cells together with the pEGFP-D2R minigene. The ratio of D2S to D2L mRNA was examined by RT-PCR. The arrow and arrowhead indicate D2L and D2S mRNA PCR products, respectively. Error bars represent the mean ± S.E. (n = 3). *, p < 0.05; **, p < 0.01 versus mock-transfected cells. HPRT, hypoxanthine-guanine phosphoribosyltransferase.