FIGURE 4.

Nova-1 protein recognizes a specific sequence of D2R exon 6. A, sequence of D2R exon 6. Mutated sequences are underlined, and each “CA” was changed to “TT” in each mutant DNA. B, EMSA using Nova-1 (300 nm) was performed with various point mutant exon 6 RNA probes (m1, m2, m3, m4, and m5). The arrow indicates the specific binding of Nova-1 to RNA probes. C, the pEGFP-D2R-WT, pEGFP-D2R-m2, or pEGFP-D2R-m5 minigene was introduced into NIH3T3 cells. The D2S/D2L mRNA ratio was examined by RT-PCR. Error bars represent the mean ± S.E. (n = 3). *, p < 0.05 versus WT. HPRT, hypoxanthine-guanine phosphoribosyltransferase.