FIGURE 6.

Nova-1 regulates alternative D2R pre-mRNA splicing via binding to D2R exon 6 directly. D2R-WT and mutant minigenes that express D2R-m2 or D2R-m5 were introduced together with hnRNP M and/or Nova-1 protein expression plasmids into NIH3T3 cells. Arrows and arrowheads indicate D2L and D2S mRNAs, respectively. The D2S/D2L mRNA ratio was analyzed by RT-PCR. Error bars represent the mean ± S.E. (n = 3). *, p < 0.05; **, p < 0.01 versus hnRNP M-transfected cells. HPRT, hypoxanthine-guanine phosphoribosyltransferase.