TABLE 1.

Primer sequences for plasmid constructs

Name	Primer sequence
T7-E6
Upstream	5′-TAATACGACTCACTATAGGGGGCAACTGTACCCACCCT-3′
Downstream	5′-CATTCTCCGCCTGTTCA-3′
E6-1
Upstream	5′-ATGCGAGCTCGGCAACTGTACCCACCC-3′
Downstream	5′-ATGCGGTACCAACGGTGCAGAGTTTCA-3′
E6-2
Upstream	5′-ATGCGAGCTCATCATGAAGTCTAATGG-3′
Downstream	5′-ATGCGGTACCCATTCTCCGCCTGTTCA-3′
T7-E6-m1
Upstream	5′-TAATACGACTCACTATAGGGGGCAACTGTACCTTCCCTG-3′
T7-E6-m2
Downstream	5′-CATTCTCCGCCTGTTCACTGGGAAACTCCCATTAGACTTCAAAATAAC-3′
T7-E6-m3
Downstream	5′-CATTCTCCGCCTGTTCACAAGGAAA-3′
T7-E6-m4
Upstream	5′-TAATACGACTCACTATAGGGGGTTACTGTACCCACCCTG-3′
T7-E6-m5
Upstream	5′-TAATACGACTCACTATAGGGGGCAACTGTACCCACCCTGAGGATTTGAAAC-3′
D2R-m2
Upstream	5′-CTCCACAGGGCAACTGTACCTTCCCTGAGGACATGAAACTC-3′
Downstream	5′-GAGTTTCATGTCCTCAGGGAAGGTACAGTTGCCCTGTGGAG-3′
D2R-m5
Upstream	5′-CATGAAACTCTGCACCGTTATTTTGAAGTCTAATGGGAGTTTCC-3′
Downstream	5′-GGAAACTCCCATTAGACTTCAAAATAACGGTGCAGAGTTTCATG-3′