FIGURE 6.

PS decreases the IR level in adipose cells. A, WT and −/− MEF cells were incubated with 0.5 mm 3-isobutyl-1-methylxanthine, 0.25 μm dexamethasone, and 10 μg/ml insulin for 6 days and maintained in 10% FBS/DMEM to 8 days. Differentiated adipocytes were fixed and stained with Oil Red O solution. B, cell lysates from the adipocytes derived from WT or −/− MEF cells were subjected to immunoblotting with anti-IR antibody. β-Actin was detected as a loading control. C, the relative band density of IR was quantitatively analyzed. The level of IR was normalized to that of β-actin.