FIGURE 5.

FetMP facilitate iron acquisition of E. coli strain ECA458 (ΔentC ΔfecABCDE ΔfeoABC ΔmntH ΔzupT) derivatives under anaerobic conditions. E. coli strains ECA612 (glmS-fetMP) (●), ECA613 (glmS-fetM) (■), ECA614 (glmS-fetP) (▴), and ECA611 (glmS-Gm) (○) were cultivated for 17 h at 37 °C anaerobically in TMM with 0.2% glucose as carbon source. Additionally, the growth medium contained the indicated concentrations of Fe(II)SO₄ (A) or Fe(III)Cl₃ (B). Turbidity at 600 nm was determined, and averages of four independent experiments with S.D. values (error bars) are shown.