TABLE 1.
Bacterial strains and plasmids used in this study
| Strains and plasmids | Relevant genotype | Reference/Source |
|---|---|---|
| E. coli strains | ||
| F11 | Extraintestinal pathogenic E. coli (ExPEC), fetMP− | Ref. 59 |
| ECA150 | W3110 ΔlacZYA | This study |
| ECA458 | W3110 ΔentC ΔfecABCDE ΔfeoABC ΔmntH ΔzupT | This study |
| ECA611 | ECA458 glmS-Gm | This study |
| ECA612 | ECA458 glmS-fetMP-Gm | This study |
| ECA613 | ECA458 glmS-fetM-Gm | This study |
| ECA614 | ECA458 glmS-fetP-Gm | This study |
| ECA615 | W3110 ΔlacZYA glmS-fetMp-lacZ | This study |
| XL1-Blue | recA1, endA1, gyrA96, thi-1, hsdR17, relA1, supE44, lac (F¢, proAB, lacIqZ_M15, Tn10) | Stratagene (La Jolla, CA) |
| EC100DTMpir-116 | F−mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ−rpsL nupG pir-116(DHFR) | Epicentre Biotechnologies (Madison, WI) |
| BL21 | F−dcm, omp, hsdS, (rB−mB−), lon−, galλ, DE3 | Novagen (Darmstadt, Germany) |
| Vectors | ||
| pGEM®-T Easy | Promega (Mannheim, Germany) | |
| pASK-IBA3 | IBA (Göttingen, Germany) | |
| pET22b(+) | Novagen | |
| pTNS1 | Ref. 15 | |
| pUC18R6K-mini-Tn7T-Gm | Ref. 15 | |
| pUC18-mini-Tn7T-Gm-lacZ | Ref. 15 | |
| Plasmids | ||
| pECD967 | pGEM-fhuFp | Ref. 19 |
| pECD1097 | pGEM-fetMp | This study |
| pECD1098 | pASK-IBA3-fetM | This study |
| pECD1099 | pASK-IBA3-fetP | This study |
| pECD1100 | pASK-IBA3-fetMP | This study |
| pECD1101 | pET22b(+)-fetP | This study |
| pECD1102 | pUC18R6K-mini-Tn7T-Gm-fetMP | This study |
| pECD1103 | pUC18R6K-mini-Tn7T-Gm-fetM | This study |
| pECD1104 | pUC18R6K-mini-Tn7T-Gm-fetP | This study |
| pECD1105 | pUC18R6K-mini-Tn7T-Gm-fetMp | This study |
| pECD1106 | pUC18R6K-mini-Tn7T-Gm-fetMp-lacZ | This study |