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. 2011 May 24;286(28):25331–25340. doi: 10.1074/jbc.M111.221754

FIGURE 3.

FIGURE 3.

Nonfunctional loop 2 mutants were rescued by a gating mutation with altered desensitization kinetics compared with the gating mutant alone. A, the K46N mutant was not functional. However, addition of V252S mutation to the K46N mutant rescued the function of the receptor. B, the K46D mutant was not functional. However, addition of V252S mutation rescued the function of the receptor. C, the ACh dose-response relationships of the rescued receptors exhibited rightward shift (EC50 = 13.40 ± 0.32 μm for K46D/V252S and EC50 = 2.04 ± 0.05 μm for K46N/V252S) when compared with that for the V252S mutant alone (EC50 = 0.32 ± 0.01 μm). Error bars are smaller than the symbols. D, the rescued mutant receptors also had faster desensitization kinetics when compared with the gating mutant alone (τV252S = 15.60 ± 0.58 s, n = 4; τV252S/K46N = 13.92 ± 0.21 s, n = 4, p < 0.05; τV252S/K46D = 12.95 ± 0.49 s, n = 3, p < 0.05).