TABLE 1.

EC₅₀ values of ACh-induced currents in the M2 mutants of α7 nAChR

Mutants	Current
	EC50	Fold change in EC50	nH	n
	μm
V246S	72.66 ± 7.78a	−3	1.28 ± 0.09	6
L247S	nonfunctional
L248S (9′)	0.43 ± 0.01a	−490	3.07 ± 0.09	3
S249S (WT)	210.52 ± 24.29	1	1.01 ± 0.06	6
L250S	98.67 ± 10.82a	−2	2.17 ± 0.27	5
T251S	114.14 ± 8.68b	−2	2.60 ± 0.30	5
V252S (13′)	0.32 ± 0.01a	−658	1.14 ± 0.14	3
F253S	157.87 ± 12.72	1	1.03 ± 0.01	6
M254S	154.18 ± 8.04	1	1.43 ± 0.14	6
L255S (16′)	7.76 ± 0.14a	−27	2.66 ± 0.12	4
L256S (17′)	3.47 ± 0.16a	−61	2.09 ± 0.24	6
V257S	30.72 ± 2.00a	−7	1.07 ± 0.09	6
A258S	266.30 ± 33.71	1	1.15 ± 0.04	5

^a p < 0.001 compared with wild type.

^b p < 0.01.