FIGURE 1.

Percoll-purified mitochondria display minimal contamination with other cellular membrane compartments. Panel A, shown are rat liver fractions. Panel B, shown are mouse liver fractions. A Western blot for Na⁺, K⁺ATPase (plasma membrane marker), LAMP-2 (lysosomal marker), calnexin (ER marker), and VDAC (mitochondrial marker) was performed by loading an equal amount (30 μg) of homogenate (H) or mitochondrial (M) protein per lane, except rat VDAC was assessed using protein loading of 15 μg per lane. The blot is representative of three independent experiments.