FIGURE 5.
Liver mitochondria from WT mouse and rat display ceramidase activity at neutral pH and contain NCDase. Panel A, mitochondria from WT mouse, NCDase KO mouse, and rat were treated and subjected to neutral ceramidase activity assay as described under “Experimental Procedures” using d-erythro-C12-ceramide as a substrate. The results represent the average ±S.D. *, p < 0.05, n = 3. Panel B, Western blot (IB) analysis shows the presence of NCDase in WT mouse and rat mitochondria but not in mitochondria from NCD KO mouse. VDAC was used as a loading control. The blot is representative of three independent experiments.