FIGURE 1.

Inhibition of HSV-1 entry by 12-mer synthetic peptides is not specific to any particular gD receptor. G1 and G2 peptides were examined for their ability to block HSV-1 infection of 3-OST-3 (panel A)-, nectin-1 (panel B)-, and Herpesvirus Entry Mediator (panel C)-expressing CHO-K1 cells. Cells were preincubated with G1, G2, or control peptide (abbreviated as Cp) at the indicated concentrations in mm or mock-treated (abbreviated as C) with 1× phosphate saline buffer for 60 min at room temperature. After 60 min, a β-galactosidase-expressing recombinant virus HSV-1(KOS)gL86 (25 pfu/cell) virus was used for infection. After 6 h, the cells were washed, permeabilized, and incubated with ONPG substrate (3.0 mg/ml) for quantitation of β-galactosidase activity expressed from the input viral genome. The enzymatic activity was measured at an optical density of 410 nm. Each value shown is the mean of three or more determinations (±S.D.).