Role of PKR in eIF2α phosphorylation in infected cells under transcriptional suppression. The Fig is adapted from Ikegami et al.[26] (A) Schematic representations of S segments of MP-12 and rMP12-PKRΔE7. (B) VeroE6 cells were independently infected with MP-12, rMP12-rLuc and rMP12-PKRΔE7 at an moi of 3, or were mock infected. Cells were immediately treated with ActD (Act) or 50 μg/ml of α-amanitin (Ama), or were untreated. Cell extracts were prepared at 16 h.p.i. for Western blot analysis. Western blot analysis showing the accumulation of eIF2α, phosphorylated eIF2α, N protein, NSs protein, Flag-PKRΔE7 and β-actin in infected VeroE6 cells.