Figure 1.

Antioxidant enzymes are reduced in bronchoalveolar lavage (BAL) of respiratory syncytial virus (RSV)-infected mice. Groups of mice were infected with RSV or sham inoculated with saline (Control) and BAL was collected at Days 1, 3, 5, and 9. BAL proteins were resolved on 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis and Western blots were performed using antibodies against superoxide dismutase (SOD) 1, SOD 2, SOD 3, catalase, and glutathione S-transferase (GST)-mu. Membranes were stripped and reprobed for β-actin as an internal control for protein integrity and loading. Lanes 1 to 4 are BAL from four control and 5 to 8 from four RSV-infected mice at each time point. Densitometric analysis of Western blot band intensities is presented in Figure E1. The figure is representative of three independent experiments, each experiment with four mice per group at each time point.