Figure 4.

Two-dimensional gel electrophoresis (2DE) of bronchoalveolar lavage (BAL) proteins reveals a global reduction in antioxidant enzyme expression after respiratory syncytial virus (RSV) infection. SYPRO Ruby–stained 2DE of BAL from (A) control and (B) RSV-infected mouse at Day 3 after infection Desalted and albumin-depleted proteins (200 μl at 1 mg/ml concentration) were fractionated over immobilized pH gradients from 3 to 10 in the horizontal dimension, followed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis in the vertical dimension. Left, migration of molecular mass standards (kDa). The spot volumes were normalized based on the total spot volume for each gel, and the control and RSV-infected samples were compared. The numbers indicate differentially expressed spots identified by tryptic peptide mass fingerprinting and listed in C.