TABLE 1.
DIFFERENTIAL EXPRESSION OF ANTIOXIDANT ENZYMES IN BAL OF MICE BY TWO-DIMENSIONAL GEL ELECTROPHORESIS
| Fold Change in RSV BAL Compared to Control |
|||||
|---|---|---|---|---|---|
| AOE | Day 1 | Day 3 | Day 5 | Day 9 | Day 25 |
| 1-Cys peroxiredoxin protein | −1.0 | −6.1 | — | −4.1 | — |
| Catalase | — | −2.5 | −2.1 | — | — |
| Cu/Zn SOD 1 | −2.3 | −3.4 | −2.0 | −2.0 | — |
| Glutathione peroxidase 1 | −1.8 | −2.3 | — | 1.3 | — |
| Glutathione S-transferase | — | — | — | −6.0 | — |
| Glutathione S-transferase omega 1 | −6.8 | −3.6 | −2.3 | −2.0 | 1.3 |
| Glutathione S-transferase, alpha 4 | −2.2 | — | — | — | — |
| Glutathione S-transferase, mu 1 | — | −4.0 | −7.0 | −1.7 | 1.4 |
| Glutathione S-transferase, mu 2 | — | −4.3 | — | 3.4 | −1.3 |
| Glutathione-disulfide reductase | — | — | — | 3 | — |
| Nonselenium glutathione peroxidase | −2.6 | — | −4.2 | −1.3 | 1.2 |
| Peroxiredoxin 6 | — | −3.1 | −3.9 | −4.1 | 1.3 |
| Peroxiredoxin 2 | 2.7 | 2.4 | −2.1 | 1.7 | — |
| Thioredoxin 1 | — | 1.5 | — | — | 1.1 |
Definition of abbreviations: AOE = antioxidant enzymes; BAL = bronchoalveolar lavage; RSV = respiratory syncytial virus; SOD = superoxide dismutase; 2DE = two-dimensional gel electrophoresis.
Shown are fold changes of spot volume/intensity in RSV-infected over control mice. Listed AOE protein spots were identified based on high-probability from peptide mass fingerprinting in MALDI-TOF-MS (Expectation score). — indicates a protein spot that was not excised from the 2DE gel for mass fingerprinting.