Table 4.

Comparison of sequence coverage and assembly error rates in assembly of single-end reads with various GC contents and depths of coverage (BCER=0.6%)

	RL	Seq	DC	SS	VC	Eu	Ed	Ve	AB	SO
	(bp)	(GC%)
SC (%)	35	D.mel (36.90)	30×	79.48	78.76	75.44	77.17	77.43	77.55	78.70
			50×	79.74	77.60	76.33	78.55	77.97	78.29	78.59
			70×	79.54	77.70	76.40	78.33	77.86	78.06	78.62
		T.bru (44.38)	30×	72.64	71.01	68.07	67.02	67.78	67.19	68.74
			50×	73.16	70.39	68.40	67.45	67.94	67.21	68.65
			70×	73.56	70.40	68.59	67.27	67.58	67.15	68.76
	75	D.mel (36.90)	30×	80.93	79.44	78.94	78.41	79.93	79.92	80.09
			50×	80.13	79.45	78.69	80.58	79.83	79.82	80.49
			70×	80.99	79.83	79.40	80.02	79.99	79.83	80.82
		T.bru (44.38)	30×	77.92	77.12	71.02	75.67	74.84	74.83	76.99
			50×	77.57	78.43	70.60	76.20	74.91	74.66	76.59
			70×	78.68	78.38	71.48	76.99	74.86	75.02	76.70
AER (%)	35	D.mel (36.90)	30×	0.31	0.27	0.34	0.23	0.28	0.26	0.32
			50×	0.39	0.29	0.36	0.33	0.29	0.23	0.32
			70×	0.32	0.24	0.38	0.26	0.23	0.26	0.39
		T.bru (44.38)	30×	0.27	0.17	0.25	0.08	0.07	0.04	0.16
			50×	0.32	0.14	0.26	0.07	0.05	0.04	0.14
			70×	0.33	0.16	0.26	0.09	0.06	0.04	0.10
	75	D.mel (36.90)	30×	0.42	0.75	0.42	0.53	0.28	0.23	0.39
			50×	0.42	0.76	0.45	0.49	0.37	0.29	0.41
			70×	0.45	0.79	0.49	0.53	0.35	0.31	0.43
		T.bru (44.38)	30×	0.63	0.67	0.47	0.59	0.46	0.42	0.66
			50×	0.56	0.84	0.52	0.46	0.49	0.48	0.63
			70×	0.65	0.88	0.48	0.53	0.46	0.49	0.67

SC, sequence coverage; AER, assembly error rate.