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. Author manuscript; available in PMC: 2012 Jun 10.
Published in final edited form as: Cytoskeleton (Hoboken). 2011 Jun 10;68(6):340–354. doi: 10.1002/cm.20516

Figure 1. Centrally located Oxidized Actin Bodies (OABs) form rapidly after hydrogen peroxide treatment.

Figure 1

Figure 1

Figure 1

Figure 1

(A) Time course of OAB formation. Cells were treated with 2.9 mM H2O2 and at the indicated times, fixed and stained with rhodamine-phalloidin. Arrows indicate OABs. Scale bars, 5 μm. (B) Central Z-sections 12 (left) and 13 (right) from a total of 25 sections that were collected at 0.3 μm intervals from cells untreated or treated for 1 h with 2.9 mM H2O2. Scale bar, 1 μm. (C) Stereo image pairs of the cells shown in (B) generated from 3D reconstructions made from Z-stacks spanning the entire cell depth. Scale bar, 1 μm. (D) Co-localization of Apb140p and Sac6p with actin in OABs. Cells expressing GFP fusions to Abp140p and Sac6p were treated with 2.9 mM H2O2 for 1 h, fixed for 10 min and stained with rhodamine-phalloidin. Scale bars, 5 μm.