Figure 6. Actin cysteines are required for efficient recovery from oxidative stress.

Representative growth curves monitored by OD₅₉₅ for wild type and mutant cells grown in (A) YPD and (B) YPD plus 2.9 mM H₂O₂. Untreated and treated cultures were seeded from starter cultures after an initial 3.5 h of logarithmic growth in YPD. Cultures were incubated and monitored in a TECAN Infinite F200. (C) Comparison of the recovery time for actin cysteine mutants. The recovery time was measured as the time interval from starting the culture (t=0) till the time each culture reached half of the OD₅₉₅ reading at the diauxic shift. To illustrate the delay in recovery of mutants, bars in the graph show the recovery times subtracted for the recovery time observed for wild type cells. Values represent averages ± S.D. from at least three different experiments. (D) Comparison of the percent of cell viability for wild type and mutant strains before addition of 2.9 mM H₂O₂ (T=0, light gray), 2 h post-H₂O₂ addition (dark gray) and at a time point between OD ₅₉₅ 0.4–0.6 after recovery (black). Values are means ± S.D.