Figure 3. Absence of ISGylation did not alter the tumor spectrum or survival in K-ras^LA2 mice.

A. Western blotting analysis showing the absence of ISGylation in lung tissues from Ube1l^−/−/K-ras^LA2 mice (lanes 6–10) compared to Ube1l^+/+/K-ras^LA2 mice (lanes 1–5). The membranes were blotted with anti-tubulin α antibody first before it was striped and reprobed with anti-ISG15 antibody. B. The incident rate of different tumor types in K-ras^LA2 moribund mice (n = 15 for both groups). C. The survival curves of Ube1l^+/+/K-ras^LA2 mice (solid line, n = 21) and Ube1l^−/−/K-ras^LA2 mice (dashed line, n = 24). Mice were observed weekly for death events and tumor development. There are no differences in survival between the two genotypes (P = 0.21, Logrank test)