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. 2011 Jun 7;105(1):74–82. doi: 10.1038/bjc.2011.170

Figure 2.

Figure 2

Effects of GLCE on the viability and proliferation of U2020 cells in vitro. (A) The colony formation efficiency of epi-pETE- and pETE-transfected U2020 cells (transient transfection). Original plates stained with Giemsa are shown. (B) Quantitative analysis of colony numbers for the epi-U2020 and control pETE-U2020 plates. The number of colonies on the control pETE-U2020 plates was set as 100%. The histogram shows the average colony numbers from triplicate experiments. Statistical analysis was performed using OriginPro 8.1 (data represent the mean±s.d.). (C) Representative multiplex RT–PCR experiment showing the expression of GLCE. (D) Proliferation of epi-U2020 and pETE-U2020 cells (Growth curve assay).