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. 2011 May 31;105(1):146–153. doi: 10.1038/bjc.2011.190

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Copyright © 2011 Cancer Research UK

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HBx suppresses the expression of miR-15a/16 by activating c-Myc in HepG2 cells. (A) qRT-PCR and (B) representative western blot results showed that HBx upregulated c-Myc transcription and translation in HepG2 cells. Relative c-Myc expression was normalised to β-actin and GAPDH. The qRT-PCR data are presented as mean±s.e. fold compared with HepG2-vc (n=3, Student's t-test). The c-Myc/GAPDH ratio is listed for the western blots. (C) The relative expression of c-Myc and miR-15a/16 was analysed by qRT-PCR at 24, 48, 72, and 96 h after the transfection of si-c-Myc into HepG2-hbx cells compared with the negative control (NC) siRNA transfectants, normalised to β-actin and U6, respectively. The data are presented as mean fold change±s.e. (^*P< 0.05, ^**P<0.01, ^***P<0.001; n=3, Student's t-test).