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. Author manuscript; available in PMC: 2011 Jul 15.
Published in final edited form as: Br J Nutr. 2008 Jun;99(Suppl 3):S37–S47. doi: 10.1017/S0007114508006831

Fig. 4.

Fig. 4

RPA analysis for Gpx1 mRNA, Gpx4 mRNA and Gapdh mRNA (A) and correlation between blood Gpx1 mRNA level and plasma Se concentration (B). A: Total RNA (10 μg) isolated from blood from Reading subjects was subjected to RPA analysis. Shown is a representative autoradiogram for RNA isolated from a human male liver (Liver), from blood from 19 Reading subjects, and from blood from male and female US volunteers. Locations of the protected Gpx1, Gpx4 and Gapdh fragments are shown. tRNA lane shows control hybridization reaction with 20 μg of yeast tRNA with all of the probes. B: Resulting Gpx1 mRNA values normalized to Gapdh mRNA levels and set to 100 %, and plotted against plasma Se values (●). Shown is the linear regression line (–), the correlation coefficient and P-value for significance. Also plotted for reference are values from two US volunteers (male: ▼, female: ■) and a simple hyperbolic fit for the Reading data (…).