Figure 3.

Local downregulation of adenosine kinase (ADK) attenuates ischemic neuronal injury in the cortex. After 60 minutes of middle cerebral artery occlusion (MCAO) and 23 hours of reperfusion, mice were killed and the brains were 2,3,5-triphenyltetrazolium chloride (TTC) stained to evaluate ischemic damage. Healthy tissue stains pink, while damaged tissue remains white. (A) Representative TTC staining of wild-type (WT, left) and fb-Adk-def (right) mice. The white arrow indicates the cortical region protected against MCAO, while the yellow arrow indicates the region of exacerbated damage in the striatum of fb-Adk-def mice. (B) Quantification of indirect infarct volume in the cortex (left panel) and striatum (right panel) of WT (white bars, n=10) and fb-Adk-def mutant (black bars, n=11) mice. Data are displayed as mean±s.e.m. ^*P<0.05 and ^**P<0.01, versus WT mice.