Figure 3.

Brain weight from mice with wild-type Pten, or homozygous and heterozygous missense mutations in Pten. (a) Wild-type (left), heterozygote (center), and homozygous mutant (right) littermate female mice at 5 weeks of age, demonstrating obvious macrocephaly in the homozygote. (b) A representative image of wild-type (left), heterozygous (center), and homozygous mutant (right) brains from littermate mice at 2 months of age. (c) Brain weight increases with dose of mutant Pten, potentially interacting with sex (males – grey, females – white). (d) Brain weight with age in Pten^M3M4/M3M4 (circles), Pten^WT/M3M4 (diamonds), and Pten^WT/WT mice (stars). Note that brain weights of heterozygotes begin to differ from wild-type mice only by 3 months of age.