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. Author manuscript; available in PMC: 2011 Jul 15.
Published in final edited form as: Annu Rev Genet. 2009;43:143–166. doi: 10.1146/annurev-genet-102108-134205

Figure 3.

Figure 3

The Arabidopsis DNA demethylase ROS1 is required for preventing transcriptional silencing of genes that are under dynamic control by DNA methylation and demethylation. (a) Dynamic control of DNA methylation level and transcription activity by DNA methyltransferase and demethylase enzymes. (b) Silencing of the RD29A-LUC transgene in the ros1-1 mutant. Left, luminescence image of the ros1-1 mutant and WT plants; right, bright field illumination of all plants. The color scale under the luminescence image shows the luminescence intensity from black (lowest) to white (highest). Silencing in the ros1 mutant can be released by mutations in any component of the RNA-directed DNA methylation pathway, supporting dynamic control of the RD29A-LUC transgene by the opposing methylation and demethylation pathways. WT, wild type Arabidopsis seedlings.