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. 2011 Jul;22(7):1213–1220. doi: 10.1681/ASN.2010101022

Figure 2.

Figure 2.

(A) The iPS colonies display a normal 46XY karyotype. (B) PCR of genomic DNA shows the integration of the target vectors in mesangial cell-derived iPS cells at passage 4. Lanes 1–7 show representative iPS cells following retroviral transduction, confirming the expression of OCT3/4, c-Myc, KLF4 and SOX2, compared to human embryonic stem (hES) cells (Lane 8) and normal cultured mesangial cells (NHMC; Lane 9). β-actin is shown as a loading control and for positive amplification. 154x218mm (600 × 600 DPI).