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. 2011 Jul;22(7):1252–1261. doi: 10.1681/ASN.2010040367

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Copyright © 2011 by the American Society of Nephrology

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Figure 5. — SOD2 and Txnrd2 were potential target genes of miR-335 and miR-34a, respectively. (A) Construction of luciferase-wild-type UTR vectors (Luc-SOD2-UTR and Luc-Txnrd2-UTR) and the corresponding luciferase-mutated UTR vectors (Luc-SOD2-mutant UTR and Luc-Txnrd2-mutant UTR). (B) The effect of miR-335 on luciferase activity in mesangial cells cotransfected with miR-335 mimic (premiR-335) and Luc-SOD2-UTR vector or Luc-SOD2-mutant UTR. *P < 0.05 versus premiR control. (C) The effect of miR-34a on luciferase activity in mesangial cells cotransfected with miR-34a mimic (premiR-34a) and Luc-Txnrd2-UTR vector or Luc-Txnrd2-mutant UTR. *P < 0.05 versus premiR control.