Figure 5.

Sulf-deficient mice demonstrate altered VEGFA distribution. Left panel: Immunogold staining on control (n = 3) and Sulf1^−/−;Sulf2^−/− kidneys (n = 3). Arrows mark VEGFA staining. The lower panels are magnifications of the upper panels. Size bars = 500 nm. Right panel: Quantification of gold particles on two control and two Sulf double mutant kidney samples. For the controls a total of 27 pictures were taken with a surface area of 3 μm² and for the mutants 20 pictures were taken from less affected areas and 15 pictures from more affected areas. Three different glomeruli were counted and per glomerulus three different capillary loops. Error bars: ±SEM. t test (two-tailed two-sample). Abbreviations: PFP, podocyte foot process; GEC, glomerular endothelial cell.