Skip to main content
. 2011 Jul 15;6(7):e21877. doi: 10.1371/journal.pone.0021877

Figure 1. Lentivirus infection drives long term and stable gene expression in primary keratinocytes.

Figure 1

A, Primary mouse keratinocytes were infected with lentivirus encoding GFP, and infection efficiency was assessed by FACS analysis at day 5 post-infection. Y-axis indicates the intensity of GFP, X-axis represents forward angle light scatter. B, Primary keratinocytes were infected with lentivirus encoding GFP or ΔNp63α and whole cell protein was collected at day 5 and 14 and analyzed for p63 expression by western blot. C, Primary keratinocyte cultures expressing lenti-GFP or lenti-ΔNp63α were fixed at 14 days post-lentiviral transduction and incubated with anti-p63 antibody, followed by a secondary antibody conjugated with Alexa-488. The cells were then stained with DAPI to visualize the cell nuclei.