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. 2011 Jul 15;6(7):e22160. doi: 10.1371/journal.pone.0022160

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Glorieux et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Maintenance of the structural integrity of the explants during in vitro cultivation was evaluated by assessing the three-dimensional organization of the explants. Epithelial thickness (A), thickness of the lamina reticularis (B) and percentages of nuclei and collagen within a region of interest of the lamina propria (C) were evaluated in explants at different time points of in vitro cultivation. Data are represented as means+SD (error bars) of triplicate independent experiments.