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. 2011 Jul 15;6(7):e22088. doi: 10.1371/journal.pone.0022088

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He et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) HAT activity of ectopically expressed p300. Plasmids pCMV-p300HA which encodes HA-tagged p300 or pcDNA3.1-p300HAT- which encodes p300 (HAT-) mutant were transfected into HeLa (left panels) or 293T cells (right panels). Western-blot was employed to determine protein expression and the HAT activity. (B) Histone H2B and H4 transcription was enhanced by p300HA but not p300 (HAT-). n = 4. (C) p300 (HAT-) mutant was unable to rescue the down-regulation of H2B transcription resulted from CBP knockdown. HeLa cells were co-transfected with siRNA and plasmid DNA as indicated. 48 hours after transfection, cells were harvested for RT-real time qPCR. Comparison between column 2 and 3 was analyzed with unpaired t test. n = 4.