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. 2011 Jul 15;6(7):e22138. doi: 10.1371/journal.pone.0022138

Figure 3. Expression of alternative TCF4 mRNAs in human tissues and brain regions.

Figure 3

(A) RT-PCR analysis of TCF4 transcripts with different 5′ exons and (B) with alternative internal splicing. Transcripts are designated as in Figure 1C. The positions of bands respective to the transcripts encoding the full-length (FL) and Δ isoforms, − and + isoforms are indicated at the left on panel B. mRNAs with longer exon 18 (+) give rise to RT-PCR product that has a unique BglII restriction site enabling discrimination from RT-PCR products amplified from mRNAs not containing the 12 bps insert (−). House-keeping gene SDHA mRNA expression is shown at the bottom of the panel. PCR with no template was performed as a negative control (neg) with each primer pair.