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. 2011 Jul 15;6(7):e22395. doi: 10.1371/journal.pone.0022395

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Gu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Cos 7 cells were transfected with 0.375 (left panel) or 0.75 (right panel) fmoles of pcDNA-AR, 25 fmoles of the report plasmid pGL3-4xARE-E4-luc (left panel) or pGL3-Probasin-luc (right panel), or 30 fmoles of pcDNA-f:GFP-p44 or pcDNA-f:GFP-p44mt. Transfected cells were grown in the presence of 10 nM R1881 for 48 hr and then harvested for luciferase assay. Values represent mean ± SD (n = 3). (B) Western blot analysis of whole-cell lysates (10 µg proteins per sample) derived from the transfected cells with the anti-p44 (top) or anti-actin (bottom) antibody as indicated. The transfected GFP-p44 fusion protein, endogenous p44 protein, and β-actin are indicated by arrows on the right. (C) Western blot of cytoplasmic and nuclear fractions of the transfected cells described in (A) with anti-FLAG, -HSP90, or anti-lumin B antibody.