Table 1.
Sequences of primers used in the amplification of renal genes by RT-PCR
| Gene | Accession no. or reference | Forward primer | Reverse primer | Size (bp) | Temp. (°C) |
|---|---|---|---|---|---|
| Collagen I | Li et al. [27] | 5′-CATAAAGGGTCATCGTGGCTTC-3′ | 5′-GTGATAGGTGATGTTCTGGGAG-3′ | ∼500 | 62 |
| Collagen IV | Li et al. [27] | 5′-GCAGGTGTGCGGTTTGTGAAG-3′ | 5′-AGCTCCCCTGCCTTCAAGGTG-3′ | 328 | 55 |
| Fibronectin | NM019143 | 5′-CAAGACCATACCTGCCGAAT-3′ | 5′-CCGTGTAAGGGTCAAAGCAT-3′ | 417 | 63 |
| AT1 | NM030985 | 5′-GAGAGGATTCGTGGCTTGAG-3′ | 5′-TAAGTCAGCCAAGGCGAGAT-3′ | 464 | 62 |
| TGF-β1 | NM021578 | 5′-GACCTGCTGGCAATAGCTTC-3′ | 5′-GACTGGCGAGCCTTAGTTTG-3′ | 468 | 58 |
| PAI-1 | M24067 | 5′-CCCTTCCAGAGTCCCATACA-3′ | 5′-CAGGCGTGTCAGCTCATTTA-3′ | 485 | 60 |
| uPA | NM013085 | 5′-CAGATCCGATGCTCTTAGCC-3′ | 5′-GCTGCTCCACCTCAAACTTC-3′ | 458 | 61 |
| β-actin | NM031144 | 5′-AGCCATGTACGTAGCCATCC-3′ | 5′-CTCTCAGCTGTGGTGGTGAA-3′ | 228 | 60 |
Temp, temperature.