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. Author manuscript; available in PMC: 2012 Jul 15.
Published in final edited form as: ACS Chem Biol. 2011 Apr 21;6(7):700–708. doi: 10.1021/cb2000296

Figure 4.

Figure 4

Importazole reversibly blocks importin-β-mediated nuclear import in living cells. (a) Schematic showing that the GFP-tagged, NLS-containing transcription factor NFAT enters the nucleus upon treatment with the ionophore ionomycin in a RanGTP- and importin-β-dependent manner. (b) HEK 293 cells stably expressing GFP-NFAT were treated with DMSO or 40 µm importazole for 1 hour prior to a 30 min treatment with ionomycin to induce nuclear import. Importazole was washed out and after 1 hour prior to ionomycin re-treatment. (c) Results were quantified as the percentage of cells with nuclear NFAT-GFP. N=3, 100 or more cells counted under each condition. Bars represent standard error.