Importazole does not inhibit Crm1-mediated nuclear export. (a) Schematic illustrating that upon removal of the ionophore ionomycin, GFP-NFAT exits the nucleus in a RanGTP- and Crm1-dependent manner. (b) Cells were treated with ionomycin to induce nuclear import of NFAT-GFP, then washed and treated with DMSO, importazole, leptomycin B, or importazole + leptomycin B for 1 hour. (c) Results were quantified as the percentage of cells with nuclear NFAT-GFP. N=3, 100 or more cells counted under each condition. Bars represent standard error.