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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Dev Dyn. 2011 Jun 17;240(8):1964–1976. doi: 10.1002/dvdy.22680

Fig.1.

Fig.1

Gene expression detection of all the genes of interest (gfap included), using (A) RT-PCR in 2% ethidium bromide agarose gel electrophoresis and (B) quantitative real-time PCR (qPCR). Levels of mRNA, measured by qPCR were normalized to β-act. Error bars indicate ± SEM. Student's t-test, p<0.05. Abbreviations: bhlhb5, basic helix-loop-helix domain containing, class B, 5; dbx1a, developing brain homeobox 1a; dmbx1a, diencephalon/mesencephalon homeobox 1a; gfap, glial fibrillary acidic protein; her4.2, hairy-related 4.2; hes5, hairy and enhancer of split 5; hoxa5a, homeo box A5a; hoxb5b, homeobox protein (hoxb5b) gene; nxph1, neurexophilin 1; plxnd1, plexin D1.