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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Cancer. 2011 Oct 1;117(19):4424–4438. doi: 10.1002/cncr.26073

Figure 4. DAC induces autophagy in Hey cells but not in SKOv3 cells.

Figure 4

(A) Hey and SKOv3 ovarian cancer cells were transfected with GFP-LC3 plasmids and treated with DAC, SAHA, or a combination at their respective IC50 concentrations. Formation of punctate GFP-LC3 spots is indicative of autophagy. (B) Hey and SKOv3 cells were treated with DAC or SAHA for 1, 3, and 5 days. After staining with acridine orange, the fraction of autophagic cells was determined by flow cytometry. (C) Hey cells were treated with DAC or SAHA, or a combination for 5 days. Cells were then harvested and processed for TEM. The arrows indicate autophagosomes. N, nucleus; M, mitochondria.