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. Author manuscript; available in PMC: 2012 Jun 21.
Published in final edited form as: Biochemistry. 2010 Dec 21;50(2):230–239. doi: 10.1021/bi101401h

FIGURE 5.

FIGURE 5

Sedimentation velocity analysis of wild-type and mutant forms of IDH. Van Holde and Weischet integral distribution plots of sedimentation coefficients (28, 29) for affinity-purified forms of IDH are shown. The top panels (open symbols) show plots for the truncated -5IDH1/IDH2 enzyme and mutant enzymes also containing a residue substitution as indicated. The bottom panels (closed symbols) show plots for the wild-type IDH1/IDH2 and mutant enzymes containing a residue substitution as indicated. Sedimentation velocity analyses were conducted at 25,000 rpm or at 60,000 rpm as indicated.