Table 1.
Sedimentation coefficients for major boundary species.
| Enzyme | 25,000 rpm |
60,000 rpm |
|||
|---|---|---|---|---|---|
| Major species (% boundary) | Sedimentation coefficient (× 1013) | Major species (% boundary) | Sedimentation coefficient (× 1013) | ||
| A | -5IDH1/IDH2 | 90 | 10.9 | 82 | 10.6 |
| -5IDH1G15D/IDH2 | 63 | 6.8 | 63 | 6.8 | |
| -5IDH1D168K/IDH2 | 73 | 6.8 | 56 | 7.0 | |
| -5IDH1G15A/IDH2 | —b | — | 50 | 9.9 | |
| -5IDH1D168A/IDH2 | 90 | 10.6 | 62 | 10.7 | |
| B | IDH1/IDH2 | 92 | 10.7 | 75 | 10.9 |
| IDH1G15D/IDH2 | 77 | 6.8 | 76 | 6.9 | |
| IDH1D168K/IDH2 | — | — | 55 | 7.0 | |
a
Sedimentation coefficients were determined from van Holde and Weischet integral distribution plots.
b
Blanks indicate broad sedimentation patterns and heterogeneity of species, precluding assignment of sedimentation coefficients.