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. 2011 Jan 6;7(3):518–531. doi: 10.1007/s12015-010-9225-3

Fig. 1.

Fig. 1

Differentiation of hES cells. A) Protocol. Arrows indicate medium renewal. B) Photomicrographs of morphological changes during differentiation. C) and D) immunofluorescence analysis of N-CAM, FOXA2, AFP, HNF4, CK8-18 and Albumin in ES-Hep and PCHH. Only overlays (FOXA2-green/AFP-red; ALB-green/CK8-18 or HNF4-red) are shown here. See Supplementary Fig. 2 for further detail