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Effects of 8-PK on NF-κB and IRF-3 nuclear translocation, IκB degradation and Akt phosphorylation in H1N1-infected A549 cells. For nuclear translocation and cytosolic IκB degradation, cells were lysed at 16 h p.i. and then protein was extracted by western blot technique as described in Section 2. While assessing for Akt phosphorylation, cells were lysed at 6 h p.i. then cytosolic protein was extracted. Similar results were obtained in four independent experiments.
