Figure 6.

Effect of wortmannin, LY294002 or 8-PK on PI3K activity in H1N1-infected A549 cells. For PIP3 production, cells were lysed at 16 h p.i. and then protein was extracted for immunoprecipitation and ELISA assay as described in Section 2. Data reported are mean ± SE of three independent experiments, each performed in triplicate. *P < .05 and **P < .01, indicates significant differences as compared with H1N1 inoculation alone. Western blot analysis of the immunoprecipitates using anti-p85 antibodies confirmed that equal amounts of p85 protein were used in the assays (b).