Figure 10.
EAS5 induced apoptosis and activated caspase-3 in NB4 cells. (a) EAS5-induced apoptosis of NB4 cells in a dose-dependent manner after 72 h of treatment. The apoptotic sub-G1 populations in the flow cytometric analysis histograms were obtained as measures of apoptosis and the percentages of them were shown below the histograms. (b) The protein level of cleaved/activated caspase-3 of NB4 cells was increased by EAS5 after 72 h of treatment. The cells were treated with PBS or EAS5 (3.75–30 μg/mL) for 72 h and then harvested for flow cytometry (a) and western blot (b) analysis. EAS5: active fraction of THL.