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The effects of THL on the PML-RARα fusion protein and DNMT1 of NB4 cells. (a) THL dose-dependently decreased the protein level of PML-RARα detected by antibodies against either RARα or PML. (b) The protein level of DNMT1 was also decreased by THL in the same manner. The cells were treated with PBS or THL (0.375–3 mg/ml) for 72 h and then harvested. Cell lysates were then prepared for western blot analysis.
