Figure 6.

The expression of effector caspase-3 in cordycepin-induced apoptosis. Cells were treated without or with 100 μM and 1 mM cordycepin for different time (0, 1, 3, 6, 12, and 24 h, resp.). Caspase-8 (16 kDa), caspase-9 (37 kDa), caspase-3 (19 kDa) and caspase-7 (20 kDa) specific bands were detected by Western blot. Immunoblot represents the observations from one single experiment repeated three times (a). The integrated optical densities (IOD) of caspase-8, -9, -3, and -7 proteins after normalization with β-actin (43 kDa) in each lane using PDI image system were demonstrated in (b), (c), (d), and (e), respectively. Each data point in the figure represents the mean ± SEM of three separate experiments. *Statistical difference compared to control (P < .05).